6R8C
HIV capsid hexamer with IP5 ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-04 |
Detector | OXFORD SAPPHIRE CCD |
Wavelength(s) | 0.978 |
Spacegroup name | P 6 |
Unit cell lengths | 90.663, 90.663, 56.997 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.690 - 1.920 |
R-factor | 0.19308 |
Rwork | 0.191 |
R-free | 0.23426 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6es8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.652 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.520 | 1.980 |
High resolution limit [Å] | 1.913 | 1.930 |
Rmerge | 0.056 | 0.620 |
Number of reflections | 20758 | 1240 |
<I/σ(I)> | 17.7 | 2.6 |
Completeness [%] | 99.3 | 90 |
Redundancy | 6.5 | 6 |
CC(1/2) | 0.998 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 270 | PEG 6K, 1M NaCl |