6R82

Crystal structure of the TLDc domain of Skywalker/TBC1D24 from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9789
Spacegroup name	P 43 21 2
Unit cell lengths	89.483, 89.483, 135.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.369 - 2.046
R-factor	0.1963
Rwork	0.195
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4acj
RMSD bond length	0.008
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.380	2.170
High resolution limit [Å]	2.046	2.046
Rmerge	0.094	0.972
Number of reflections	35238	5519
<I/σ(I)>	19.2
Completeness [%]	99.8	99.2
Redundancy	15.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	28% PEG 3350, 0.1 M Hepes pH 7.5 and 0.6M ammonium citrate