6R7E

N-terminally reversed variant of FimA E. coli with alanine insertion at position 20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	87.100, 87.100, 163.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.550 - 1.790
R-factor	0.1765
Rwork	0.175
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nkt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.550	43.550	1.750
High resolution limit [Å]	1.700	7.620	1.700
Rmerge	0.138	0.029	7.969
Rmeas	0.145	0.031	8.377
Number of reflections	26259	340	1892
<I/σ(I)>	10.63	54.85	0.21
Completeness [%]	99.3	99.1	98.2
Redundancy	10.094	9.026	10.268
CC(1/2)	0.999	0.999	0.122

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.1	298	45% NH4SO2 and 0.1 M Sodium Malonate buffer pH 3.1