6R7B

Crystal structure of Csx1 in complex with cyclic oligoadenylate cOA4 conformation 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9785
Spacegroup name	I 21 21 21
Unit cell lengths	107.080, 117.680, 357.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.290 - 3.120
R-factor	0.183
Rwork	0.180
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qzt
RMSD bond length	0.010
RMSD bond angle	1.260
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	178.630	3.460
High resolution limit [Å]	3.120	3.120
Number of reflections	24510	1226
<I/σ(I)>	11.7	1.4
Completeness [%]	93.0
Redundancy	11.6	13.1
CC(1/2)	0.990	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1M TRICINE PH=8.0, 0.35M NACL, 28% PEG1000, 10% GLYCEROL