6R6K
Structure of a FpvC mutant from pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.270, 53.560, 84.390 |
| Unit cell angles | 90.00, 93.16, 90.00 |
Refinement procedure
| Resolution | 43.740 - 2.100 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r3z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.740 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.061 | |
| Number of reflections | 40831 | 6474 |
| <I/σ(I)> | 9.12 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | MPG 2K, 100 mM MES |
