6R60
asymmetric antiparallel assembly of two 5-bladed beta-propeller fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 39.754, 107.519, 179.187 |
| Unit cell angles | 90.00, 94.40, 90.00 |
Refinement procedure
| Resolution | 37.200 - 1.750 |
| R-factor | 0.20011 |
| Rwork | 0.198 |
| R-free | 0.24035 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ymu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.530 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.088 | 0.454 |
| Number of reflections | 74436 | 11645 |
| <I/σ(I)> | 9.17 | 1.92 |
| Completeness [%] | 98.1 | 95.3 |
| Redundancy | 3.31 | 3.4 |
| CC(1/2) | 0.995 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Magnesium chloride, 0.1 M HEPES pH 7, 15%(w/v) PEG 4000 |
