6R5X
8-bladed beta-propeller formed by four 2-bladed fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.235, 119.673, 84.153 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.300 - 1.700 |
| R-factor | 0.19982 |
| Rwork | 0.198 |
| R-free | 0.23857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ymu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.547 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.048 | 0.570 |
| Number of reflections | 31066 | 4842 |
| <I/σ(I)> | 17.8 | 2.32 |
| Completeness [%] | 99.3 | 97.4 |
| Redundancy | 4.31 | 4.38 |
| CC(1/2) | 0.999 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M Sodium acetate 0.1 M TRIS.HCl pH 8.5 30 %(w/v) PEG 4000 |
