6R5J
New MAX Effector from Magnaporthe oryzae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-18 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 73.341, 24.760, 68.613 |
Unit cell angles | 90.00, 100.88, 90.00 |
Refinement procedure
Resolution | 34.589 - 1.380 |
R-factor | 0.1442 |
Rwork | 0.143 |
R-free | 0.17050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NMR model |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.590 | 1.430 |
High resolution limit [Å] | 1.380 | 1.380 |
Number of reflections | 59102 | 2877 |
<I/σ(I)> | 14 | |
Completeness [%] | 93.7 | |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0,1M Hepes pH 7.5 1.6M Ammonium Sulfate 1,6M PEG 1000 |