6R5J

New MAX Effector from Magnaporthe oryzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-18
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	73.341, 24.760, 68.613
Unit cell angles	90.00, 100.88, 90.00

Refinement procedure

Resolution	34.589 - 1.380
R-factor	0.1442
Rwork	0.143
R-free	0.17050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NMR model
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.590	1.430
High resolution limit [Å]	1.380	1.380
Number of reflections	59102	2877
<I/σ(I)>	14
Completeness [%]	93.7
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0,1M Hepes pH 7.5 1.6M Ammonium Sulfate 1,6M PEG 1000