6R5I
The crystal structure of the Glycoside Hydrolase BglX from P. aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979270 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.079, 73.701, 81.534 |
| Unit cell angles | 65.58, 73.91, 69.70 |
Refinement procedure
| Resolution | 19.930 - 1.800 |
| R-factor | 0.16826 |
| Rwork | 0.168 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tf0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.570 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.100 | 0.240 |
| Number of reflections | 124434 | 6074 |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 96.2 | |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 50mM BIS TRIS pH6.5 + 30% pentaerythritol ethoxylate + 50mM ammonium sulfate |
