6R4E

Crystal structure of human GFAT-1 in complex with Glucose-6-Phosphate and L-Glu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	153.847, 153.847, 166.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.134 - 2.353
R-factor	0.1751
Rwork	0.174
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zj3
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XDS (Jan 26, 2018)
Phasing software	PHENIX ((dev_2499: ???))
Refinement software	PHENIX ((dev_2499: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.134	2.440
High resolution limit [Å]	2.350	2.350
Number of reflections	82721	7933
<I/σ(I)>	18.1
Completeness [%]	99.6
Redundancy	12.9
CC(1/2)	0.999	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 8.25, 0.4 M Potassium sodium tartrate, 20 % PEG3350