6R48
Crystal structure of LPOR (Synechocystis) complexed with NADPH at 1.87A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 56.960, 72.890, 155.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.570 - 1.870 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.472 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.570 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.097 | 0.881 |
| Number of reflections | 51836 | 51836 |
| <I/σ(I)> | 11.8 | 2.1 |
| Completeness [%] | 95.2 | 86.5 |
| Redundancy | 5.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 10MM ZNSO4,0.2M LI3CITRATE, 30% PEG3350, EVAPORATION, TEMPERATURE 293K |
