6R47
The structure of pistol ribozyme bound to magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 94 |
Detector technology | PIXEL |
Collection date | 2017-03-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 61 |
Unit cell lengths | 94.309, 94.309, 96.031 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 81.670 - 3.100 |
R-factor | 0.1762 |
Rwork | 0.176 |
R-free | 0.18020 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | iMOSFLM |
Data scaling software | pointless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.670 | 3.310 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.067 | |
Number of reflections | 8853 | 1592 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 99.7 | |
Redundancy | 3.9 | |
CC(1/2) | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.01 M Magnesium sulphate heptahydrate 0.05 M Sodium cacodylate trihydrate pH 6.0 1.8 M Lithium sulphate monohydrate |