6R3Z
Structure of the SBP FpvC in complex with Ni2+ ion from P. aeruginosa in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.570, 62.690, 90.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.260 - 1.650 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | model soved by SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.051 | |
| Number of reflections | 30063 | 4251 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 97.8 | |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 16% PEG 6K, 0.1 M MES |
