6R3A
BACTERIOPHAGE SPP1 MATURE CAPSID PROTEIN
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.3 |
| Detector | GATAN K2 BASE (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4 |
| Number of Particle | 200 |
| Software | EMAN2,IMAGIC,Coot,Leginon,CTFFIND |
| Symmetry | I |
| CTF correction | PHASE FLIPPING ONLY |
