6R2I
Crystal structure of the SUN1-KASH5 6:6 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9282 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 80.163, 80.163, 177.620 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.989 - 1.541 |
R-factor | 0.1505 |
Rwork | 0.149 |
R-free | 0.16830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6r15 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.076 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.810 | 1.570 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.068 | 1.515 |
Rmeas | 0.070 | 1.551 |
Rpim | 0.015 | 0.329 |
Number of reflections | 49458 | 7234 |
<I/σ(I)> | 23.5 | 2.2 |
Completeness [%] | 97.5 | 100 |
Redundancy | 21.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.12M 1,6-Hexanediol; 0.12M 1-Butanol 1,2-Propanediol (racemic); 0.12M 2-Propanol; 0.12M 1,4-Butanediol; 0.12M 1,3-Propanediol; 0.1M Imidazole pH 6.5 ; 0.1M MES (acid) pH 6.5; 18% Glycerol; 18% PEG 4K |