6R27
Crystallographic superstructure of the photosensory core module (PAS-GAF-PHY) of the bacterial phytochrome Agp1 (AtBphP1) locked in a Pr-like state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 240.218, 240.218, 160.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.890 - 3.400 |
| R-factor | 0.25713 |
| Rwork | 0.255 |
| R-free | 0.29701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hsq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.443 |
| Data reduction software | XDS (Nov 1, 2016) |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.890 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.101 | 0.438 |
| Rmeas | 0.128 | 0.557 |
| Rpim | 0.079 | 0.341 |
| Number of reflections | 32444 | 4659 |
| <I/σ(I)> | 10.5 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.9 | 5 |
| CC(1/2) | 0.990 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 20-23% PEG 3350, 50 mM NaCl and 100 mM Tris/Cl, in 20% Sucrose as cryoprotectant |
