6R26

The photosensory core module (PAS-GAF-PHY) of the bacterial phytochrome Agp1 (AtBphP1) locked in a Pr-like state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97952
Spacegroup name	I 4 2 2
Unit cell lengths	174.445, 174.445, 79.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.840 - 3.110
R-factor	0.21895
Rwork	0.216
R-free	0.27618
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i5l
RMSD bond length	0.004
RMSD bond angle	1.402
Data reduction software	XDS (Nov 1, 2016)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.840	3.320
High resolution limit [Å]	3.110	3.110
Rmerge	0.083	0.356
Rmeas	0.093	0.397
Rpim	0.041	0.175
Number of reflections	11232	1987
<I/σ(I)>	16.3	5
Completeness [%]	99.3	98.7
Redundancy	9.4	9.4
CC(1/2)	0.997	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	291	20-23% PEG 3350, 50 mM NaCl and 100 mM Tris/Cl, in 30% PEG 3350 as cryoprotectant