6R1J

Structure of the soluble AhlC triple head mutant of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	103
Detector technology	PIXEL
Collection date	2018-07-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97951
Spacegroup name	P 61 2 2
Unit cell lengths	88.538, 88.538, 290.975
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.680 - 1.920
R-factor	0.23658
Rwork	0.234
R-free	0.27796
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h2e
RMSD bond length	0.009
RMSD bond angle	1.613
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.860	1.900
High resolution limit [Å]	1.900	1.900
Number of reflections	52765	2550
<I/σ(I)>	16.1
Completeness [%]	100.0
Redundancy	18.7
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289.15	0.1M imidazole pH 8.0, 10% PEG 8000