6R1I
Structure of porcine Aichi virus polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 170.448, 170.448, 231.339 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.128 - 2.634 |
R-factor | 0.1916 |
Rwork | 0.191 |
R-free | 0.22190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | polyalanine model based on 4nz0 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.764 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.130 | 2.730 |
High resolution limit [Å] | 2.634 | 2.634 |
Number of reflections | 59076 | 5790 |
<I/σ(I)> | 33.96 | |
Completeness [%] | 100.0 | |
Redundancy | 21.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.7M (NH4)2SO4, 1.7% (v/v) PEG400, 15% (v/v) glycerol, 85 mM HEPES pH 7.5 |