6R1H
Crystal structure of the LRR ectodomain of the receptor kinase SOBIR1 from Arabidopsis thaliana.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-02 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000027 |
Spacegroup name | P 65 |
Unit cell lengths | 82.246, 82.246, 109.863 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.500 - 1.550 |
R-factor | 0.1732 |
Rwork | 0.172 |
R-free | 0.18770 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.500 | 1.650 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmeas | 0.074 | 2.730 |
Number of reflections | 60378 | 9692 |
<I/σ(I)> | 25 | 0.84 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 18.5 | 12.6 |
CC(1/2) | 1.000 | 0.510 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 25 % (v/v) 3,350 1M LiCl 0.1 M sodium acetate pH 5.5 |