6R13
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 7f
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.871, 58.547, 88.283 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.790 - 1.650 |
R-factor | 0.19907 |
Rwork | 0.198 |
R-free | 0.22371 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v2y |
RMSD bond length | 0.011 |
RMSD bond angle | 1.713 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.790 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.070 | 0.820 |
Number of reflections | 36299 | 5754 |
<I/σ(I)> | 20.72 | 2.35 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 12.9 | 13.2 |
CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.4 M Ammonium phosphate |