6R0L
Histone fold domain of OsGhd8/NF-YC7 in I2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.967700 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 65.836, 34.102, 67.081 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 44.770 - 2.700 |
| R-factor | 0.25851 |
| Rwork | 0.258 |
| R-free | 0.27638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g49 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.210 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.356 | |
| Number of reflections | 38743 | 3887 |
| <I/σ(I)> | 3.6 | 2.1 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.967 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 20% PEG 4000, 0.1 M sodium acetate pH 5.0, 0.2 M ammonium acetate |
