6R0H

Glycogen Phosphorylase b in complex with 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION NOVA
Temperature [K]	293
Detector technology	CCD
Collection date	2017-05-10
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.5419
Spacegroup name	P 43 21 2
Unit cell lengths	128.287, 128.287, 116.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.710 - 2.500
R-factor	0.16174
Rwork	0.159
R-free	0.21278
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.651
Data reduction software	CrysalisPro
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.710	2.610
High resolution limit [Å]	2.500	2.500
Number of reflections	32589	3644
<I/σ(I)>	10.3	1.6
Completeness [%]	96.1	89.7
Redundancy	3.7	2.6
CC(1/2)	0.977	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.8	289	10 mM BES buffer