6R04
T. cruzi FPPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999910 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 57.650, 57.650, 397.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.930 - 1.470 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dwg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS (20180126) |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.264 | 1.495 |
| High resolution limit [Å] | 1.469 | 1.469 |
| Rmerge | 0.060 | 2.813 |
| Rmeas | 0.062 | 2.896 |
| Rpim | 0.015 | 0.687 |
| Total number of observations | 1242005 | |
| Number of reflections | 68742 | 3327 |
| <I/σ(I)> | 20.8 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.1 | 17.6 |
| CC(1/2) | 1.000 | 0.380 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 80 mM MES: 8.5 mM ZnSO4: 19.42 % PEG MME 550: 15% glycerol |
