6QZV
DPP9 bound to a dipeptide (MP) from the N-terminus of BRCA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.00002 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 119.516, 117.219, 163.401 |
Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
Resolution | 43.470 - 3.000 |
R-factor | 0.28871 |
Rwork | 0.286 |
R-free | 0.34517 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6eoq |
RMSD bond length | 0.004 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.470 | 3.190 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.130 | 0.300 |
Number of reflections | 76534 | 13092 |
<I/σ(I)> | 5.9 | 3 |
Completeness [%] | 87.8 | 88.8 |
Redundancy | 2.65 | |
CC(1/2) | 0.980 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 20 mg/ml of protein, 10% PEG 8000, 25% glycerol, 0.16 M calcium acetate, 0.08 M cacodilate pH 6.25. Drops are set in a 1:1 ratio. |