6QYY

The crystal structure of head fiber gp8.5 N base in bacteriophage phi29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	63
Detector technology	PIXEL
Collection date	2016-03-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9798
Spacegroup name	P 1 21 1
Unit cell lengths	55.212, 65.070, 118.874
Unit cell angles	90.00, 99.09, 90.00

Refinement procedure

Resolution	41.790 - 1.800
R-factor	0.1846
Rwork	0.182
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.866
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.790	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	76833
<I/σ(I)>	13.5
Completeness [%]	99.3
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M HEPES (pH 6.0), 0.15 Ammonium sulfate, 4% (v/v) DMSO