6QYF

4'-phosphopantetheinyl transferase PptAb from Mycobacterium abscessus at pH 4.6 with Mg2+ and CoA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.967700
Spacegroup name	P 21 21 2
Unit cell lengths	63.080, 82.226, 55.996
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 1.400
R-factor	0.145
Rwork	0.145
R-free	0.15790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u89
Data reduction software	TRUNCATE
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	56325	4954
<I/σ(I)>	22.2
Completeness [%]	97.0
Redundancy	6.7
CC(1/2)	0.999	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	285	0.1 M Na acetate 30 % PEG 4K 0.2 M NH4 acetate