6QYC
Crystal structure of MtrC from Shewanella baltica OS185
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.72001 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 90.520, 291.500, 87.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.200 - 2.290 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22830 |
| Structure solution method | SAD |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.520 | 2.350 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.112 | 1.887 |
| Rmeas | 0.117 | 1.986 |
| Rpim | 0.033 | 0.612 |
| Total number of observations | 1317009 | |
| Number of reflections | 104279 | 7485 |
| <I/σ(I)> | 17 | |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 12.6 | 10.2 |
| CC(1/2) | 0.999 | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.4 M Sodium Acetate, 5% PEG 6000, 5% PEG 8000, 5% PEG 10000 |
