6QWT

Sicinivirus 3Dpol RNA dependent RNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 42 21 2
Unit cell lengths	92.509, 92.509, 161.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 2.300
R-factor	0.1917
Rwork	0.190
R-free	0.22390
RMSD bond length	0.003
RMSD bond angle	0.578
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	31996
<I/σ(I)>	13.4
Completeness [%]	99.9
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	12,5 % PEG 1000, 12,5 % PEG 3350, 12,5 % (RS)-2-methyl-2,4-pentanediol, 0,2 M 0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M (RS)-1,2-propanediol, 0.2 M 2-propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol and 0,1 M MES/Imidazole pH 6.5