6QWP

Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0679
Spacegroup name	P 42 21 2
Unit cell lengths	261.570, 261.570, 256.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.560 - 3.400
R-factor	0.23203
Rwork	0.230
R-free	0.27534
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.196
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.560	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.025
Number of reflections	110605
<I/σ(I)>	5.9
Completeness [%]	95.7
Redundancy	3.4
CC(1/2)	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	15 % tacsimate, 0.1 M HEPES pH 7.0, 12 % (w/v) PEG 3350