6QWP
Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0679 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 261.570, 261.570, 256.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.560 - 3.400 |
R-factor | 0.23203 |
Rwork | 0.230 |
R-free | 0.27534 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.196 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.560 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.025 | |
Number of reflections | 110605 | |
<I/σ(I)> | 5.9 | |
Completeness [%] | 95.7 | |
Redundancy | 3.4 | |
CC(1/2) | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15 % tacsimate, 0.1 M HEPES pH 7.0, 12 % (w/v) PEG 3350 |