6QWF

The Transcriptional Regulator PrfA-A94V mutant from Listeria Monocytogenes in complex with a 30-bp operator PrfA-box motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96770
Spacegroup name	P 43 21 2
Unit cell lengths	78.438, 78.438, 265.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.557 - 2.700
R-factor	0.2268
Rwork	0.225
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lej
RMSD bond length	0.003
RMSD bond angle	0.509
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.560	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.097	1.367
Rpim	0.026	0.363
Number of reflections	23582	2304
<I/σ(I)>	21.2	3.2
Completeness [%]	99.9	100
Redundancy	26.8	28.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	Droplets of 4 microl protein-DNA solution (ratio 1:1.3) in 20 mM Tris-HCl pH 8.0, 50 mM NaCl, and 1 mM EDTA at 6 mg per ml were mixed with 2 microl reservoir solution consisting of 8% PEG 8000, 100 mM sodium citrate pH 4.6, 100 mM Magnesium acetate and 20% glycerol.