6QVP
Crystal structure of the peptidoglycan-binding domain of SiiA from Salmonella enterica
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.334, 58.360, 65.828 |
| Unit cell angles | 93.88, 94.00, 119.00 |
Refinement procedure
| Resolution | 19.964 - 1.900 |
| R-factor | 0.1892 |
| Rwork | 0.186 |
| R-free | 0.24620 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.114 | 0.540 |
| Number of reflections | 57795 | |
| <I/σ(I)> | 7.5 | 2.3 |
| Completeness [%] | 95.9 | 93.8 |
| Redundancy | 1.7 | |
| CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 292 | 0.2 M Sodium phosphate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350 |
