6QVI
Crystal structure of competence-associated pilin ComZ from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.860, 121.860, 212.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.790 - 2.720 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.551 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.790 | 2.790 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.058 | |
| Number of reflections | 49699 | |
| <I/σ(I)> | 18.1 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.8 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M potassium thiocyanate, 0.1 M Bis-Tris propane pH 6.5 and 20% w/v PEG 3350 |
