6QVF
TT_C0855 competence pilin from Thermus thermophilus HB27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.588, 137.815, 59.739 |
| Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
| Resolution | 68.910 - 1.440 |
| R-factor | 0.1518 |
| Rwork | 0.151 |
| R-free | 0.18930 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.435 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.000 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.059 | |
| Rmeas | 0.070 | |
| Rpim | 0.038 | |
| Number of reflections | 154421 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 86.5 | 59.3 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M ammonium nitrate, 0.1 M Bis-Tris propane pH 8.5, and 18% v/v PEG Smear High |
