6QVB
CryoEM structure of the human ClC-1 chloride channel, CBS state 3
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.34 |
| Number of Particle | 477729 |
| Software | Gautomatch,RELION,PHENIX,FREALIGN,Gctf,UCSF Chimera |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING ONLY |
