6QV5
Crystal structure of the CHAD domain from the plant Ricinus communis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-31 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.999859 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 116.427, 116.427, 119.248 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.650 - 2.300 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.24200 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.890 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.650 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.172 | 3.677 |
Number of reflections | 40129 | 6493 |
<I/σ(I)> | 15.5 | 1 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 21.2 | 21.5 |
CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 3 M NaCl, 0.1 M Bis-Tris pH 6.0 |