6QUZ

Structure of ATPgS-bound outward-facing TM287/288 in complex with sybody Sb_TM35

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	165.340, 77.110, 207.010
Unit cell angles	90.00, 112.49, 90.00

Refinement procedure

Resolution	48.840 - 3.210
R-factor	0.244
Rwork	0.243
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.840	48.840	3.290
High resolution limit [Å]	3.200	14.330	3.200
Rmerge	0.249	0.036	0.965
Rmeas	0.258	0.038	1.001
Number of reflections	63765	959	421
<I/σ(I)>	8.91	50.92	3.03
Completeness [%]	79.6	96	7.2
Redundancy	13.589	13.416	14.309
CC(1/2)	0.998	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1M Sodium acetate, 0.035M NaCl, 11.5% (w/v) PEG 6000