6QUK
Protein crystallization by ionic liquid hydrogel support: glucose isomerase grown by using ionic liquid hydrogel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97779 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.900, 92.598, 99.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.980 - 1.580 |
| R-factor | 0.16824 |
| Rwork | 0.167 |
| R-free | 0.19449 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oad |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.635 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | Sir2014 |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.900 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.099 | 0.664 |
| Rmeas | 0.115 | 0.791 |
| Rpim | 0.070 | 0.492 |
| Number of reflections | 103356 | 7748 |
| <I/σ(I)> | 11.1 | 3.4 |
| Completeness [%] | 95.6 | 97.4 |
| Redundancy | 4.2 | 4.1 |
| CC(1/2) | 0.995 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | protein 26mg/m in HEPES 10mM pH 7 and MgCl2 1mM Reservoir: (NH4)2SO4 1.5M |
