6QUH

GHK tagged GFP variant crystal form II at 1.34A wavelength

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.34
Spacegroup name	P 2 21 21
Unit cell lengths	53.476, 99.307, 102.011
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.160 - 1.500
R-factor	0.1442
Rwork	0.142
R-free	0.18750
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.407
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.310	1.530
High resolution limit [Å]	1.500	1.500
Rpim	0.044	0.524
Number of reflections	87711	8625
<I/σ(I)>	12.1
Completeness [%]	100.0
Redundancy	12.3
CC(1/2)	0.992	0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Sosium Acetate pH 5.4, 30%MPD, 0.1M Cacl2