6QUG
GHK tagged MBP-Nup98(1-29)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.3776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.910, 286.780, 110.780 |
| Unit cell angles | 90.00, 92.01, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.700 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.22640 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rpim | 0.048 | 0.411 |
| Number of reflections | 141017 | 14015 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.8 | |
| CC(1/2) | 0.998 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG550, PEG20,000, MOPS, HEPES, sodium Phosphate, sodium nitrate, ammonium sulphate |
