6QUC

Truncated beta-galactosidase III from Bifidobacterium bifidum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-14
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	118.500, 119.683, 144.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 - 2.300
R-factor	0.1884
Rwork	0.188
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gm8
RMSD bond length	0.003
RMSD bond angle	0.651
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.040	0.382
Number of reflections	91305	9057
<I/σ(I)>	8.5	1.7
Completeness [%]	99.0	99
Redundancy	2	2
CC(1/2)	0.995	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	25% PEG 550mme 100 mM Imidazole