6QUB
Truncated beta-galactosidase III from Bifidobacterium bifidum in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972423 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.781, 54.592, 143.357 |
| Unit cell angles | 90.00, 107.72, 90.00 |
Refinement procedure
| Resolution | 34.139 - 1.950 |
| R-factor | 0.1695 |
| Rwork | 0.169 |
| R-free | 0.21460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.742 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.143 | 0.617 |
| Number of reflections | 119733 | 12079 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.996 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 23% PEG1500 10mM galactose 10 mM Tris:HCL |
