6QU6

Adenovirus Serotype 26 (Ad26) in complex with sialic acid, pH4.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91587
Spacegroup name	P 21 3
Unit cell lengths	85.731, 85.731, 85.731
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.500 - 1.030
R-factor	0.13689
Rwork	0.136
R-free	0.14792
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fjn
RMSD bond length	0.012
RMSD bond angle	1.754
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	1.060
High resolution limit [Å]	1.030	1.030
Rmerge	0.053
Rmeas	0.055
Number of reflections	103975
<I/σ(I)>	0.219
Completeness [%]	97.7
Redundancy	14.9
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.1 M MIB [Malonic acid, Imidazole, Boric acid], 25 % w/v PEG 1500.