6QU1

Crystal structure of the KAP1 RBCC domain in complex with the SMARCAD1 CUE1 domain at 3.7 angstrom resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.92
Spacegroup name	P 41 21 2
Unit cell lengths	64.496, 64.496, 287.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.431 - 3.700
R-factor	0.3036
Rwork	0.300
R-free	0.34640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h3a
RMSD bond length	0.003
RMSD bond angle	0.678
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.431	4.140
High resolution limit [Å]	3.700	3.700
Rmerge	0.235	2.081
Rpim	0.106	0.805
Number of reflections	7157	1948
<I/σ(I)>	7.3	1.4
Completeness [%]	99.7	99.4
Redundancy	9.2	7.3
CC(1/2)	0.995	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	25 % PEG 3350, 0.1 M HEPES pH 7.5