6QTU
Crystal structure of Arabidopsis WD40 domain in complex with a BBX transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.679, 54.992, 102.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.480 - 1.300 |
| R-factor | 0.1429 |
| Rwork | 0.141 |
| R-free | 0.17590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5igo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.069 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 1.340 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmeas | 0.034 | |
| Number of reflections | 67449 | |
| <I/σ(I)> | 16.86 | |
| Completeness [%] | 98.7 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 5 mg/mL of COP1 supplemented with 3 to 10 fold molar excess in peptide was mixed with two-fold (v/v) more mother liquor (1:2 ratio; protein:buffer) containing 1.25 M sodium malonate pH 7.5. |
