6QTR

Crystal structure of a mutant Arabidopsis WD40 domain in complex with a transcription factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-06
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	48.260, 55.059, 103.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.740 - 1.370
R-factor	0.122
Rwork	0.120
R-free	0.16130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5igo
RMSD bond length	0.007
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.740	1.420
High resolution limit [Å]	1.370	1.370
Rmeas	0.027
Number of reflections	57218
<I/σ(I)>	22.6
Completeness [%]	97.4
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	5 mg/mL of COP1 supplemented with 3 to 10 fold molar excess in peptide was mixed with two-fold (v/v) more mother liquor (1:2 ratio; protein:buffer) containing 2 M (NH4)2SO4 and 0.1 M HEPES pH 7.4 or 0.1M Tris pH 8.5.