6QTO
Crystal structure of an Arabidopsis WD40 domain in complex with a transcription factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-26 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.293, 54.873, 103.535 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.770 - 1.270 |
R-factor | 0.1184 |
Rwork | 0.117 |
R-free | 0.14640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5igo |
RMSD bond length | 0.008 |
RMSD bond angle | 1.144 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.770 | 1.320 |
High resolution limit [Å] | 1.270 | 1.270 |
Number of reflections | 73002 | |
<I/σ(I)> | 16.12 | |
Completeness [%] | 99.9 | |
Redundancy | 21.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 5 mg/mL of COP1 supplemented with 3 to 10 fold molar excess in peptide was mixed with two-fold (v/v) more mother liquor (1:2 ratio; protein:buffer) containing 2 M (NH4)2SO4 and 0.1 M HEPES pH 7.4 or 0.1M Tris pH 8.5 |