6QTB
Crystal structure of Rea1-MIDAS/Ytm1-UBL complex from Chaetomium thermophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-31 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.87290 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.553, 83.176, 77.686 |
Unit cell angles | 90.00, 117.72, 90.00 |
Refinement procedure
Resolution | 36.580 - 1.890 |
R-factor | 0.1912 |
Rwork | 0.190 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6QT8 and 5EM2 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.494 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.620 | 1.900 |
High resolution limit [Å] | 1.840 | 1.840 |
Number of reflections | 73123 | |
<I/σ(I)> | 13.33 | |
Completeness [%] | 100.0 | |
Redundancy | 13.3 | |
CC(1/2) | 0.999 | 0.664 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 3 M NaCl and 0.1 M Tris (pH 8.5) |