6QT9
Cryo-EM structure of SH1 full particle.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.8 |
| Number of Particle | 16185 |
| Software | ETHAN,RELION,cisTEM,SerialEM,PHENIX,CTFFIND,Rosetta,Coot |
| Symmetry | C532 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
