6QSR
The Dehydratase Heterocomplex ApeI:P from Xenorhabdus doucetiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.680, 47.770, 42.680 |
Unit cell angles | 90.00, 104.65, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.850 |
R-factor | 0.20212 |
Rwork | 0.199 |
R-free | 0.23804 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3esi |
RMSD bond length | 0.007 |
RMSD bond angle | 1.294 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.069 | 0.470 |
Number of reflections | 23313 | |
<I/σ(I)> | 11 | 2.7 |
Completeness [%] | 95.9 | 97.6 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M HEPES, 30% PEG 6000 |